2D NOESY Experiment二维相敏NOESY测的是小于5A的质子间的NOE效应. N.B. - For molecules (in organic solvents) with MW from 1000 - 3000, the ROESY experiment may be more appropriate, as the NOEs for molecules of this size may be null, or zero. 实验设置: 在第一区采一个氢谱，然后输入mf(1,3)，把谱图和参数转移到第三区 输入 dg. 对于标准 NOESY，调节参数如下: ss=2 | sfrq=already set | tpwr = listed at console | tn=H1 | pw = 1H 90° pulse width listed at console | at = set by spectrometer | d1* = 1H relaxation delay (1-2 * 1H T1) | np=2048 | presat='n' | sw = 1H 谱的sw | mix=0.2 to 1.2 (see below) | d2=0 | nt* =8的整数倍 | tof =1H 谱的tof | sw1 = 1H 谱的sw | ct = set by computer | ni* = 64的整数倍 | dm='n' | | dp='y' | | hs='n' | | sspul='n' | | phase=1,2 | *改变ni, nt, 和d1 到你需要的实验时间 (see below). | temp=25 su --设置温度为25度 |
***关掉旋转*** ga开始采样
对于大分子mix取较小的时间，对于小分子，mix取较大时间 垂直方向(非采样的f1维)的分辨率为 ：sw1/2ni.
对于 sw1=4400 和 mix=0.5，典型的设置值如下：
d1 |
ni |
nt | time (hr:min) | vert. res (no 0 fill) | |
d1 |
ni |
nt | time (hr:min) | vert. res (no 0 fill) |
1.5 |
128 |
8 |
1:18 |
17.2 Hz | |
1.5 |
128 |
64 |
10:17 |
17.2 Hz |
1.5 |
256 |
8 |
2:37 |
8.6 Hz | |
1.5 |
256 |
64 |
20:42 |
8.6 Hz |
1.5 |
512 |
8 |
5:15 |
4.3 Hz | |
1.5 |
512 |
64 |
41:56 |
4.3 Hz |
1.5 |
128 |
16 |
2:34 |
17.2 Hz | |
2.25 |
128 |
8 |
1:42 |
17.2 Hz |
1.5 |
256 |
16 |
5:11 |
8.6 Hz | |
2.25 |
256 |
8 |
3:30 |
8.6 Hz |
1.5 |
512 |
16 |
10:29 |
4.3 Hz | |
2.25 |
512 |
8 |
6:59 |
4.3 Hz |
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[此贴子已经被作者于2006-11-27 21:38:42编辑过] - u1 s- {( f& M+ t( R
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