* S7 [6 A" R7 P. ]3 A# |- @+ {
8 h- q2 g; v2 m% [' d' T
Tune the probe for proton NMR
; v0 D3 V9 E, t3 @: @% a5 b
Run H-standard spectra in Exp1
. P1 v ]8 n& i1 K$ P4 D( [7 R f
create second data set (EXP2), In “eda” select ns=1pulprog=zg : Acquire and process the data (zg， efp) (this represent 90` pulse)
g5 ]9 h9 F# Z# ~& d3 m% {
Localize a peak in the center of the window, define it (DP1) in ased, try doubling pulse (p1) and acquire the data : zg efp. if the signal is null: this is 180` pulse if not try slightly different p1 pulse until null is found
发表于 2008-6-18 15:59:32
