|
|
发表于 2010-7-18 23:49:41
|
显示全部楼层
|
1) atma 1H (Tunning+Maching)' v! j% }; C3 ?( A2 S- @
2) take a normal 1H spectrum with ns = 1; rga , etc' a! t( \) I- r: ~4 a: ~
3) ft and phase it manually or just type apk- z2 v6 |/ V1 n$ m) i
4) change pulprog zg30 to zg, using eda or select AcquPars icon2 j+ u$ @' s0 v+ {* p; d. q
5) change the p1 value to (p1 plus or minus 0.1 or 0.2 and so on) *4, P; C' P9 o: ?6 B/ D
6) check the p1 value, (it should be below 50u) ->type p1 return5 l8 m0 f, ~. O7 b
7) zg again
2 A0 W( a4 [. J( s* E9 \8) fp (this moment do not phase the spectrum anyway)# [& Y; z* Z# U J3 r8 {
9) if the peaks appear mostly positive, reduce p1 value, and do step 7 and step 8 again
* I8 t& q2 E6 {% O U6 G4 ^% n10) if the peaks appear mostly negative, increase p1 value, do step 7 and step 8 again0 ~1 `+ r/ i, Z# n, P
11) while the intensity of the peaks appear mostly half positive and half negative, then it is the correct 90 pulse width *4
) {2 \4 f& a$ I" W12) divide p1 value by 4 (this is the value which can be used in 2D expts for 1H 90 pulse hard pulse).7 @4 ]" _$ E3 t$ V# e2 W
13) change pulprog = zg30, ns = 16, p1 =(90pw) start the 1H experiment( Reset to Normal Mode )) D T7 e3 K8 `* \6 p; |- O( c
Note :If no appropriate value is find at all, start the small value of p1=3u,with pulprog = zg again , then check that the phase is positive or not. It should be positive, else, try again from step 5 to step 12.
. O- \: G+ V6 q/ Y! G |
|
发表于 2006-11-15 20:44:53
